Purified anti-Phosphotyrosine

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

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SPECIFICATIONS

Catalog Number

309302

Size

100 microg

Applications

FC, ICC, IF, IP, WB

Hosts

Mouse

Clone

PY20

Antigen

KLH-conjugated phosphotyrosine

Gene Id

5800

Isotype

Mouse IgG2b, kappa

Clonality

Monoclonal

Dilutions

Western blotting, suggested working dilution(s): Use1-2 microg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored at -20C.

Alternative Names

Regulatory Status

RUO