Purified anti-PLK-1

PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The Poly6185 antibody recognizes human PLK-1 and has been shown to be useful for Western blotting and immunoprecipitation.

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SPECIFICATIONS

Catalog Number

618501

Size

50 microl

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6185

Antigen

Recombinant (partial)

Gene Id

10769

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended to use sensitive techniques such as immunoprecipitation-Western blot analysis.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13, PLK 1

Regulatory Status

RUO