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Purified anti-PLK-1 Phospho (Thr210)

Purified anti-PLK-1 Phospho (Thr210)

Cat no: 618601

Supplier: BioLegend
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PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The Poly6186 antibody recognizes human phosphorylated PLK-1 (Thr210) and has been shown to be useful for Western blotting. The Poly6186 antibody cross-reacts with an unknown kinase in non-mitotic cells. To increase specificity, it is recommended that the Poly6186 antibody be used for Western blotting after immunoprecipitation with a pan-specific PLK-1 antibody (clone 3F8).
Catalogue number: 618601
Reactivities: Human
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 50 microl
Clone: Poly6186
Gene: 10769
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store frozen at -20 C.
Antigen: Modified peptide
Isotype: Rabbit Polyclonal
Clonality: Polyclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: -20 C
Alternative names: Serine/Threonine protein kinase PLK, PLK, Serine-threonine protein kinase 13, PLK 1
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