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Purified anti-PTBP1

Purified anti-PTBP1

Cat no: 630101

Supplier: BioLegend
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The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.
Catalogue number: 630101
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 25 microg
Clone: 3H7
Gene: 5725
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5ml antibody dilution buffer for each mini-gel. For IHC, use a 15 microg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: Recombinant human PTBP1 (full length)
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, 57 kDa RNA-binding protein PPTB-1, PTB
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