Purified anti-Rb

Cat no: 614602

Supplier: BioLegend

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Description
Additional Information

Rb (retinoblastoma 1) is a 105 kD nuclear protein containing RB-associated protein A and B domains. Rb acts as a transcriptional negative regulator of G1-phase cell cycle progression, and sequesters E2F and other transcription factors. Rb is unphosphorylated and inactive in G0, and becomes active upon hypophosphorylation by cyclinD-cdk4/6 in early G1. Rb is inactive with hyperphosphorylated by cyclinE-cdk2 late G1. Hypophosphorylated Rb form complexes with E2F, HDAC, cyclinD-cdk4/6, cyclinE-cdk2, PU 1, ATF 2, UBF, Elf 1, and c-Abl. The Poly6146 antibody recognizes the N-terminal region of human and mouse Rb and has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence staining.

Catalogue number:	614602
Reactivities:	Human, Mouse
Hosts:	Rabbit
Applications:	Western Blot
Size:	200 microl
Clone:	Poly6146
Gene:	5925
Dilutions:	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000-1:10,000 dilutions. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store frozen at -20 C.
Antigen:	Human Rb (N terminal, 100 amino acids)
Isotype:	Rabbit Polyclonal
Clonality:	Polyclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	-20 C
Alternative names:	Retinoblastoma 1