Purified anti-SIRT1

Cat no: 634301

Supplier: BioLegend

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Description
Additional Information

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors. The Poly6343 antibody has been shown to be useful for western blotting and immunoprecipitation of human SIRT1 protein.

Catalogue number:	634301
Reactivities:	Human
Hosts:	Rabbit
Applications:	Immunoprecipitation, Western Blot
Size:	50 microl
Clone:	Poly6343
Gene:	23411
Dilutions:	Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use10microl per 5 ml antibody dilution buffer for each mini-gel.It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	The antibody solution should be stored undiluted at 4C. DO NOT FREEZE..
Antigen:	Peptide mapping to the carboxy terminal domain of human SIRT1.
Isotype:	Rabbit Polyclonal
Clonality:	Polyclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, hSIRT1, hSIR2