Purified anti-Sp1

The sequence-specific transcription factor Sp1 binds GC box sequences in the promoters of a variety of genes. The 97 kD Sp1 transcription factor contains two zinc finger domains that determine sequence specificity of binding to the 5'-GGGGCGGGGC-3'. This transcription factor regulates nucleoside, nucleotide and nucleic acid metabolism and many other "housekeeping" genes. Sp1 can be modified by phosphorylation by the upstream kinase ERK1 and has been shown to interact with a large number of proteins. The transcriptional activity of Sp1 has been shown to be disrupted in early Huntington's disease. The Poly6247 antibody recognizes human, mouse, and rat Sp1 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

624702

Size

500 microl

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6247

Antigen

Peptide mapping to internal domain of human Sp1

Gene Id

6667

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. DO NOT FREEZE.

Alternative Names

Transcription factor Sp1, SP1

Regulatory Status

RUO