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Puromycin Dihydrochloride (Achromycin, Stillomycin, Stylomycin, Antibiotic CL 13900, Antibiotic 3123L, Antibiotic P638, Bacterenomycin)

Cat no: P9200

Puromycin Dihydrochloride (Achromycin, Stillomycin, Stylomycin, Antibiotic CL 13900, Antibiotic 3123L, Antibiotic P638, Bacterenomycin)

Puromycin allows the selection of cells expressing the puromycin-N-acetyl-transferase (pac). The expression of pac gene confers puromycin resistance to transfected cells. Puromycin selection works for gram-positive bacteria and animal cells. Fungi and gram-negative bacteria are resistant due to low permeability of the antiboitic. Puromycin is an aminomucleoside antibiotic produced by Streptomyces alboniger. It is a powerful inhibitor of protein synthesis. It is a structural analog of the 3' end of animoacyl tRNA. When puromycin is added to an in vitro system containing all the necessary machinery for protein synthesis, protein synthesis stops and incomplete peptide chains are released from the ribosomes. Each such chain has puromycin covalently attached to one end.\n\nAppearance: White to off white powder\n\nSolubility: Colorless, clear, complete\n\nRecommended Dilution: \n1-10ug/ml\n\nMoisture: (same/less than) 12%\n\nStorage and Stability:\nStore at -20C. Stable for 12 months.

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SPECIFICATIONS

Catalog Number

P9200

Size

100mg

Purity

(same/more than)98%

References

1. Nathans, D., Antibiotics, 1: 239 (1967). 2. Exp. Cell Res., 58: 401 (1969). 3.

Alternative Names

(3

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