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Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-tag  (Cytosolic Inorganic Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)

Cat no: P9542-50C

Pyrophosphatase, Inorganic, Recombinant, E.coli, aa1-176, His-tag (Cytosolic Inorganic Pyrophosphatase, Pyrophosphate PhosphohydrolaPse)

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. \n\nSource:\nRecombinant corresponding to aa1-176 of E.coli ppa, fused with His-tag at N-terminus, expressed in E.coli.\n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE \nAAKAEIVASF ERAKNK\n\nMolecular Weight: \n~21.9kD (196aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9542-50C

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8, 1mM DTT, 50mM sodium chloride, 10% glycerol.

Purity

~95% (SDS-PAGE)

References

Harold FM., et al (1966) Bacteriol Rev 30(4):772

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