Pyruvate Dehydrogenase alpha (PDHa) ELISA Kit

Cat no: ABIN415450

Supplier: Antibodies-online

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Pyruvate Dehydrogenase alpha (PDHa) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Alpha (PDHa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Alpha (PDHa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Alpha (PDHa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN415450
Size:	96 tests
Antigen:	Pyruvate Dehydrogenase alpha (PDHa)
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero standard added by their three standard deviations.
Assay range:	78.125-5000 pg/mL
Sample type:	Serum,Plasma,Tissue Homogenate,Cell Lysate,Biological Fluids

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Pyruvate Dehydrogenase alpha (PDHa) ELISA Kit

Cat no: ABIN415450

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