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Pyruvate Kinase BioAssay(TM) Kit

Cat no: P9545-02

Pyruvate Kinase BioAssay(TM) Kit

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer\nof a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of\npyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of\nglycolysis which causes the disease known as pyruvate kinase deficiency. This kit provides a\nsimple, direct and automation-ready procedure for measuring pyruvate kinase activity in various\nbiological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP\nwere catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by\npyruvate oxidase to produce color (at lambda = 570nm) and fluorescence (at Ex/Em = 535/587nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1mU/ml pyruvate kinase.\n\nKit Components:\nPK Assay Buffer 1x25ml\nOxiRed(TM) Probe lyophilized\nDMSO (anhydrous) 1x400ul\nPK Enzyme Mix lyophilized\nPK Substrate Mix lyophilized\nPK Positive Control (~18mU) lyophilized\nPyruvate Standard (100nmol/ul) 1x100ul.\n\nStorage and Stability:\nStore the kit at -20 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9545-02

Size

100Tests

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