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Pyruvate Kinase, Muscle (PKM2) BioAssay(TM) ELISA Kit (Human)

Cat no: 027783

Pyruvate Kinase, Muscle (PKM2) BioAssay(TM) ELISA Kit (Human)

Pyruvate Kinase, Muscle (PKM2) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PKM2 in human serum, plasma, tissue homogenates and other biological fluids.\n\nDetection Range:\n78-5000pg/ml\n\nSensitivity:\n33pg/ml\n\nIntra-Assay: \nCV<10% \n\nInter-Assay: \nCV<12%\n\nKit Components:\n1. Microtiter Plate, 1x96 wells\n2. Standard, (lyophilized), 2x1vial\n3. Detection Reagent A, 1x120ul\n4. Detection Reagent B, 1x120ul\n5. TMB Substrate, 1x9ml\n6. Wash Buffer (30X), 1x20ml\n7. Standard Diluent, 1x20ml\n8. Assay Diluent A, 1x12ml\n9. Assay Diluent B, 1x12ml\n10. Stop Solution, 1x6ml\n\nStorage and Stability:\nStore Microtiter plate, Standard, Detection Reagent A and Detection Reagent B at -20 degrees C upon receipt. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.пїЅ \n\nAssay Procedure Summary:\n1. Prepare all reagents, samples and standards; \n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C. \n4. Aspirate and wash 3 times. \n4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C. \n5. Aspirate and wash 5 times.\n6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37 degrees C. \n7. Add 50ul Stop Solution. Read at 450nm immediately.

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SPECIFICATIONS

Catalog Number

027783

Size

96Tests

Additional Info

Human

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