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Pyruvate Kinase, Tumor Type M2, Recombinant, Human

Cat no: P9545-31A

Pyruvate Kinase, Tumor Type M2, Recombinant, Human

Recombinant human M2PK is expressed in E. coli with an amino-terminal hexahistidine tag.\n\nPyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n \nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9545-31A

Size

10ug

Applications

WB

Form

Supplied as a liquid in 1x Laemmli Buffer (25mM Tris-HCl pH6.8, 50mM DTT, 1% SDS, 0.1% bromophenol blue, 2.5% glycerol).

Purity

Single band on Western blot.

References

Luftner D. et al. (2000) Anticancer Res. 20(6D): 5077-82.

Alternative Names

M2PK, PKM2, Pyruvate kinase 3 isoform 1

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