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Pyruvate Kinase, Tumor Type M2, Recombinant, Human

Cat no: P9545-31C

Pyruvate Kinase, Tumor Type M2, Recombinant, Human

MTAP (Methylthioadenosine phosphorylase) is important in polyamine metabolism and for the salvage of both adenine and methionine. It is involved in the metabolism of MeSAdo (methylthioadenosine), a by-product of the synthesis of the polyamines spermidine and spermine. MTAP is deficient in many cancers because the MTAP gene is co-deleted with the tumor suppressor p16.\n\nSource: \nRecombinant human PKM2, full length, with N-terminal His tag, expressed in E. coli.\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilutions: Optimal dilutions to be determined by the researcher.\n\nEnzyme Activity: \nA 50ul PKM2 reaction is conducted in a buffer containing 50mM Tris, pH 7.4, 10mM MgCl2, 50mM KCl, 2mM ADP, 10mM phosphoenolpyruvate (PEP) at RT for 15 minutes. ATP production is detected using Kinase-Glo(R) Luminescent Kinase Assay Platform. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

P9545-31C

Size

20ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 30% glycerol.

Purity

~90%

References

1. Wong T.S. et al., Int. J. Cancer 123 (2), 251 257 (2008). 2. Christofk H.R. et al., Nature 452 (7184), 181 186 (2008).

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