Mass spectrometry (MS)-based proteomics is the primary sensitive analytical tool that can be used to determine the abundance of proteins and peptides in complex biological mixtures. Quantitative results can be used to understand different biological and pathological processes. Quantitative proteomics combined with liquid chromatography-mass spectrometry (LC-MS) is always the main method for the quantification of proteins and peptides. Existing quantitative methods in the proteomic range can be divided into.