The Quick-Stick Ligation Kit provides rapid ligation for sticky-ended and blunt-ended DNA fragments. Ligation is achieved in 5 minutes at room temperature. The kit is based on an improved enzyme activity which delivers fast, efficient ligation at room temperature.The kit enables rapid ligation of fragments from most sources, including PCR fragments, plasmids, cosmids, genomic, phage and viral DNA. The time saving is significant. The Quick-Stick Ligation Kit can provide 100% ligation of sticky-ended fragments of between 100ng and 1ug of DNA of Lambda/Hind III or 80% ligation of blunt-ended fragments for the same amount of DNA of Lambda/EcoR V. The kit can also provide complex multi-fragment ligations. Using this kit, the process will only take 5 minutes, as compared with traditional methods, which require 16 hours at 16 C.