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RAB14, Recombinant, Human, aa1-215, His-tag (Ras-related protein Rab-14, FBP, RAB-14)

Cat no: R0009-16

RAB14, Recombinant, Human, aa1-215, His-tag (Ras-related protein Rab-14, FBP, RAB-14)

RAB14 is a member of the RAB protein family, low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane bound organelles. This protein is thought to be involved in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus. It is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments. Purified by using conventional chromatography.\n\nSource:\nRecombinant corresponding to aa1-215 of human RAB14, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMATAPYN YSYIFKYIII GDMGVGKSCL LHQFTEKKFM ADCPHTIGVE FGTRIIEVSG QKIKLQIWDT AGQERFRAVT RSYYRGAAGA LMVYDITRRS TYNHLSSWLT DARNLTNPNT VIILIGNKAD LEAQRDVTYE EAKQFAEENG LLFLEASAKT GENVEDAFLE AAKKIYQNIQ DGSLDLNAAE SGVQHKPSAP QGGRLTSEPQ PQREGCGC\n\nMolecular Weight: \n~26.3kD (238aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R0009-16

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride, 1mM DTT, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

Junutula JR. et al. (2004) Mol Biol Cell. 15:2218-29. Olkkonen V.M. et al. (1993) J. Cell Sci. 106:1249-61.

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