RAB34, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. This protein is involved in protein transport, specifically the redistribution of lysosomes to the peri-Golgi region. In fibroblasts, this protein was colocalized with actin to the membrane ruffles and membranes of relatively large vesicles adjacent to the ruffles.
Source:
Recombinant corresponding to aa1-259 from human RAB34, fused to His-tag at N-terminal expressed in E.coli.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MNILAPVRRD RVLAELPQCL RKEAALHGHK DFHPRVTCAC QEHRTGTVGF KISKVIVVGD LSVGKTCLIN RFCKDTFDKN YKATIGVDFE MERFEVLGIP FSLQLWDTAG QERFKCIAST YYRGAQAIII VFNLNDVASL EHTKQWLADA LKENDPSSVL LFLVGSKKDL STPAQYALME KDALQVAQEM KAEYWAVSSL TGENVREFFF RVAALTFEAN VLAELEKSGA RRIGDVVRIN SDDSNLYLTA
SKKKPTCCP
Molecular Weight:
~31.2kD (MALDI-TOF)
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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