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RAB35, Recombinant, Human, aa1-201, His-tag (Ras-related Protein Rab-35, GTP-binding Protein RAY, Ras-related Protein Rab-1C, RAB1C, RAY)

RAB35, Recombinant, Human, aa1-201, His-tag (Ras-related Protein Rab-35, GTP-binding Protein RAY, Ras-related Protein Rab-1C, RAB1C, RAY)

Cat no: 029693

Supplier: United States Biological
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RAB35, is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Consistent with a key requirement for Rab35-regulated recycling during cell division, inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis. Source: Recombinant corresponding to aa1-201 from human RAB35, fused to His-tag at N-terminal expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C Molecular Weight: ~25.2kD (MALDI-TOF) Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 029693
Size: 50ug
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.15M sodium chloride, 1mM DTT.
Purity: ~90% (SDS-PAGE)
References: 1. Kouranti I., et al. (2006) Curr. Biol. 16:1719-1725 2. Marat A.L., et al. (2010) J. Biol. Chem. 285:10627-10637

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