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Rabbit Anti-AKT1/2/3 monoclonal antibody, clone TU59-10

Cat no: CABT-L674

Rabbit Anti-AKT1/2/3 monoclonal antibody, clone TU59-10

Rabbit Anti-AKT1/2/3 monoclonal antibody for WB, ICC/IF, IHC, IP, FC. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB? or RacPK-?) and Akt 3 (also designated PKB? or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-? tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

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SPECIFICATIONS

Catalog Number

CABT-L674

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Conjugates

Unconj

Clone

TU59-10

Target

AKT1/2/3

Gene Id

841

Isotype

IgG

Clonality

Monoclonal

Storage Temp

Store at +4В°C after thawing. Aliquot store at -20В°C or -80В°C. Avoid repeated freeze / thaw cycles.

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