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RAC1, Recombinant, Human (Ras-related C3 botulinum Toxin Substrate 1, Cell Migration-inducing Gene 5 Protein, Ras-like Protein TC25, p21-Rac1, TC25, MIG5)

Cat no: R0400-01B

RAC1, Recombinant, Human (Ras-related C3 botulinum Toxin Substrate 1, Cell Migration-inducing Gene 5 Protein, Ras-like Protein TC25, p21-Rac1, TC25, MIG5)

Rho-family GTPases, including Cdc42, Rac1 and RhoA, regulate actin cytoskeleton and cell adhesion and play a central role in establishing cell polarisation. They also play an important role in cell growth and differentiation as well as transcriptional regulation. Migrating vertebrate cells in tissue culture display a characteristic polarization of the actin cytoskeleton. The dynamic organization of the actin cytoskeleton provides the force for cell motility and is regulated by small GTPases of the Rho family, in particular Rac1, RhoA and Cdc42. Rac1 (Ras-related C3 botulinum toxin substrate 1) is a small (~21kD) GTPase, and is a member of the Rac subfamily of the family Rho family of GTPases. Rac can be activated by extracellular signals through various types of membrane receptors including receptor tyrosine kinases such as the PDGF receptor. Rac executes its biological functions through activating downstream effectors, such as PAK (p21-activated kinase). Rac plays critical roles in a wide variety of cell functions including cell cycle control, regulation of gene expression, activation of NADPH oxidase of phagocytic cells, actin cytoskeletal reorganization, axonal guidance and cell migration.\n\nSource:\nRecombinant corresponding to aa1-189, with N-GST tag, from human Rac1, expressed in E.coli.\n\nMolecular Weight:\n~48kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R0400-01B

Size

200ug

Form

Supplied as a lyophilized powder from 50mM HEPES, pH8.0, 10mM GSH. Reconstitute with sterile dH2O PBS.

Purity

~95% (SDS-PAGE)

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