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RAF, Recombinant, Human, GST-Tag (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog)

Cat no: R0495-05A

RAF, Recombinant, Human, GST-Tag (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog)

The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.\n\nSource: \nRecombinant human A-RAF, aa282-end, with N-terminal GST tag, containing the mutations YY301-302DD, expressed in Baculovirus infected Sf9 cell expression system. \n\nMolecular Weight: 63.6kD\n\nSpecific Activity: \n50nM aRAF yields 8% conversion of 1uM ATP using MEK1 (K97R) as substrate.\n \nReaction Buffer: \n10mM HEPES (pH7.4), 5mM MgCl2, 10uM MnCl2, 1mM DTT, 3uM Naorthovanadate, 1uM ATP. \n\nApplication: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

R0495-05A

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.

Purity

(same/more than)80%

References

1. Yokoyama,T. et al J. Cell Biol. 177 (5), 781- 793 (2007). 2. Ewing,R.M. et al. Mol. Syst. Biol. 3, 89 (2007).

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