RAF1, Active, Recombinant, Human (raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase)

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.\n\nSource:\nRecombinant protein corresponding to human RAF1 expressed in Sf9 insect cells.\n\nMolecular Weight: \n~63.0kD\n\nBiological Activity:\nThe specific activity of RAF1(EE) was determined to be ~6000 nmol/min/mg in a coupled assay as per activity assay protocol.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167952

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~85% (SDS-PAGE)

References

1.Rapp, U. et al: Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Nat. Acad. Sci. 80: 4218-4222, 1983. 2. Li, P. et al: Raf-1: a kinase currently without a cause but not lacking in effects. Cell 64: 479-482, 1991.