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Raf1, Recombinant, Human, GST-Tag

Cat no: R0495-01H

Raf1, Recombinant, Human, GST-Tag

Human RAF1 (GenBank Accession No. NM_002880), aa306-648, containing the mutations Y340D and Y341D, expressed in an Baculovirus infected Sf9 cell expression system. \n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\n\nSpecific Activity: As reported. Typical value:~160 pmol/min/ug. \nAssay conditions: 10uM ATP, 1uM MEK1 (K97R); 1hour\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

R0495-01H

Size

10ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4mM KCl, 16mM glutathione, 20% glycerol and 3mM DTT.

Purity

(same/more than) 80%

References

1. Lamberti,A. et al Cell Death Differ. 14 (5), 952-962 (2007)\n2. Yu,F. et al. PLoS Pathog. 3 (3), E44 (2007)

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