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Ral Activation Assay Kit, BioAssay(TM)

Cat no: R1035-61

Ral Activation Assay Kit, BioAssay(TM)

Applications:\nAssays for Ral activation and detection.\n\nQuantity:\n30 assays\n\nKit Components:\nRal BP1 (Ral Assay Reagent), agarose:\n300ug of protein in 600ul of a 50% agarose slurry in 50mM Tris, pH 7.5, 200mM NaCl, 1% NP40, 1mM PMSF, 1mM aprotinin prior to the addition of glycerol to 50%.\n\nAnti-Ral A (mouse monoclonal IgG2a):\nOne vial containing 75ug of IgG2a in 300ul of 20mM sodium phosphate pH 7.5, 150mM NaCl, 1.5mM sodium azide. 1mg/ml BSA with glycerol added to 50%.\n\nRal Activation Assay Buffer, 5X:\nTwo vials, each containing 18ml of 5X Ral Activation Assay Buffer (RAB): 250mM Tris, pH 7.5, 1M NaCl, 5% NP-40, 50mM MgCl2, 2.5mM dithiothreitol.\n\n100X GTPS, 10mM:\nOne vial containing 50ul of 10mM GTPS, 100X stock, in sterile water. Non-hydrolyzable analog of GTP. Sufficient to label 5ml of cell lysates.\n\n100X GDP, 100mM:\nOne vial containing 50ul of 100mM GDP, 100X stock, in sterile water. GDP (Guanosine 5'-Diphosphate) for in vitro labeling of G-proteins in the inactive form. Sufficient to label 5ml of cell lysates.\n\nStorage and Stability: Components are stable for 1 year at -20 degrees C from the date of shipment.

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SPECIFICATIONS

Catalog Number

R1035-61

Size

1Kit

References

de Rooij, J. and J.L. Bos, Oncogene 14: 623

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