RAMP2, Human, Control Peptide (Receptor Activity Modifying Protein2)

Control Peptide for R1060-05B and R1060-05.\n\nA 20aa Peptide mapping at the middle and a 9 aa peptide mapping near the C-terminus of human RAMP2 .\n\nThe calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175 aa; rat 182 aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nEAntibody Blocking: 5-10ug per 1ul R1060-05B or per 1ug R1060-05 \nELISA: 1ug/ml. \nOptimal dilutions to be determined by the researcher. \nOptimal dilutions to be determined by the researcher.\n\nSpecies sequence homology: \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1060-05A

Size

50ug

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. McLatchie LM et al (1998) Nature 393, 333-339. 2. Nagae T et al (2000) BBRC 270, 89-93. 3. Chakravarty p et al (2000) Br J. Pharmacol. 130, 189-195. 4. Aldecoa A et al (2000) FEBS Lett. 471, 156-160. 5. Husmann, K et al (2000); Mol Cell Endocrinol (2000) 162, 35-43

Additional Info

The peptides are 50% and 89% conserved in mouse and rat RAMP2, respectively