RANGER DNA Polymerase is a hot-start enzyme, possessing 5'-3' DNA polymerase and 3'-5' proofreading exonuclease activities, thus offering both high-fidelity and enhanced specificity. The polymerase is supplied with an industry-leading novel buffer system, specifically formulated and validated to the unique properties of RANGER. RANGER is an easy-to-use high-performance enzyme specifically designed to amplify templates of 10kb upwards with extreme sensitivity (Fig. 1). Owing to its antibody-based hot-start property, RANGER DNA Polymerase reactions can be setup at room temperature and has the added advantage of avoiding unwanted non-specific amplification and primer-dimer formation. This new hot-start enzyme from Bioline is supplied with 5x RANGER Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, delivering superior amplification. RANGER is highly suitable for all PCR applications of long templates, including targeted re-sequencing using next-generation sequencers.