RANKL, Soluble, Recombinant, Rat (Tumor Necrosis Factor Ligand Superfamily Member 11, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, Receptor Activator of Nuclear Factor kappa-B Ligand, TNF-related Activation-induced Cytokine, TRANC

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naпїЅve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4kD polypeptide comprising the TNF homologous region of RANKL (174aa).\n\nSource:\nRecombinant protein corresponding to rat Soluble RANK Ligand, expressed in E.coli.\n\nBiological Activity:\nDetermined by its ability to induce osteoclast formation in RAW264.7 cells using a concentration range of 5-10ng/ml.\n\nEndotoxin:\n<0.1ng/ug (1EU/ug)\n\nAA Sequence:\nPAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts