Rantes BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Rantes in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nRantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10kD and, after cleavage of the signal peptide, approximately 8kD. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP30882\n\nEntrez Gene ID:\n20304\n\nKit Components:\n96-well plate pre-coated with anti-Rantes, Mouse\nLyophilized recombinant Rantes, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144025

Size

96Tests

References

1. Liu, H.; Chao, D.; Nakayama, E. E.; Taguchi, H.; Goto, M.; Xin, X.; Takamatsu, J.; Saito, H.; Ishikawa, Y.; Akaza, T.; Juji, T.; Takebe, Y.; and 9 others. Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc. Nat. Acad. Sci. 96: 4581-4585, 1999.\n2. Schall, T. J.; Jongstra, J.; Dyer, B. J.; Jorgensen, J.; Clayberger, C.; Davis, M. M.; Krensky, A. M. A human T cell-specific molecule is a member of a new gene family. J. Immun. 141: 1018-1025, 1988. \n