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RAP, Recombinant, Rat (Receptor Associated Protein)

Cat no: R1115-15A

RAP, Recombinant, Rat (Receptor Associated Protein)

RAP binds with high affinity to certain members of the low-density lipoprotein (LDL) receptor family such as LDL receptor-related protein (LRP), gp330/megalin, and the very low-density lipoprotein (VLDL) receptor and, once bound to these receptors, antagonizes their ligand binding ability. Despite the presence of a putative signal sequence on RAP, this molecule is not secreted but remains cell-associated. RAP is contained primarily within the endoplasmic reticulum and Golgi compartments, with only a trace found at the cell surface and within the endosomal compartments. Genetic deletion of RAP in mice has no effect on LRP mRNA levels but does lead to incomplete processing of LRP in the brain and liver resulting in decreased LRP antigen levels in these organs.\n\nRat his: RAP:c-myc fusion partner.\n\nApplications:\nSuitable for use as protein standard in 1D and 2D SDS gel electrophoresis, immunoblotting and receptor-binding studies. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short term only. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -70 degrees C. Reconstituted product is stable for 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1115-15A

Size

100ug

Applications

WB

Form

Supplied as a lyophilized powder from TBS, pH 7.5, 0.1% BSA, 0.09% sodium azide. Reconstitute with 100ul ddH2O.

Purity

(same/more than)95% pure (SDS-PAGE)

References

1. Bajari, T.M., et al., (1998), \"A minimal binding domain of the low density lipoprotein receptor family\", Biol. Chem., 379, 1053-1062.

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