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RAP1A, aa1-181, Recombinant, Human (Ras-related Protein Rap-1A, C21KG, G-22K, Krev-1, Ras-related Protein Krev-1, KREV1)

Cat no: R1120-19

RAP1A, aa1-181, Recombinant, Human (Ras-related Protein Rap-1A, C21KG, G-22K, Krev-1, Ras-related Protein Krev-1, KREV1)

Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% aa identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st aa: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.\n\nSource: \nRecombinant corresponding to aa1-181 of human HRAS, 6x His tagged, expressed in E.coli.\n\nAA Sequences: \nMGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD \nLVRQINRKTP VEKKKPKKKS C\n\nMolecular Weight: 22.8kD (201aa), confirmed by MALDI-TOF \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1120-19

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 5mM DTT, 200mM sodium chloride, 40% glycerol.

Purity

~85% (SDS-PAGE).

References

1. Han, L., et al. (1995) Mol Cell Biol. 15 (3): 1318-23. 2. Rebhun, J F, et al. (2000) J Biol Chem. 275 (45): 34901-8.

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