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RAPD Kit (Random Amplified Polymorphic DNA), BioGenomics(TM), Kit-01

RAPD Kit (Random Amplified Polymorphic DNA), BioGenomics(TM), Kit-01

Cat no: R1125-01

Supplier: United States Biological
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Each kit contains 20 individual 10-mer primers suitable for use in genetic mapping and DNA fingerprinting. In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown. Genomic DNA from different individuals gives different PCR products, allowing the identification of DNA polymorphisms that can be used to identify different individuals or as genetic markers. The number of PCR products generated from each genomic DNA sample depends on the primer sequence, the genomic DNA sequence, and the genome size. Assuming that the priming sites of a 10mer RAPD primer are randomly distributed throughout a genome, the theoretical number of PCR products is approximately 2.5x10e9 xG, where G is the size of the haploid genome in base pairs. Using this calculation, a haploid genome of 2x10e9bp, for example, is expected to give about 5 PCR products. This prediction is in close agreement with experimental results. Vial-01: 5
Catalogue number: R1125-01
Size: 1Kit
References: 1. Grundmann, et al., J. Clin. Microbiology 35(12): 3071-3077 (1997). 2. Berg, D., et al., Methods in Molecular and Cellular Biology 5: 13-24 (1994). 3. Welsh, J. and McClelland, M., Nucleic Acids Res. 18(24): 7213-7218 (1990). 4. Williams, J.G., et al., Nucleic Acids Research 18: 6531-6535 (1990). 5. Micheli, M., et al., (1997). In Fingerprinting Methods Based on Arbitrarily Primed PCR, pp. 55-63. Springer-Verlag Press, NY. 6. Graves, L and Swaminathan, B., (1993). In Diagnostic Molecular Microbiology Principles and Applications, pp. 617-621. ASM Press, Washington, DC.

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