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RAPD Kit (Random Amplified Polymorphic DNA), BioGenomics(TM), Kit-04

RAPD Kit (Random Amplified Polymorphic DNA), BioGenomics(TM), Kit-04

Cat no: R1125-04

Supplier: United States Biological
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Each kit contains 20 individual 10-mer primers suitable for use in genetic mapping and DNA fingerprinting. In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown. Genomic DNA from different individuals gives different PCR products, allowing the identification of DNA polymorphisms that can be used to identify different individuals or as genetic markers. The number of PCR products generated from each genomic DNA sample depends on the primer sequence, the genomic DNA sequence, and the genome size. Assuming that the priming sites of a 10mer RAPD primer are randomly distributed throughout a genome, the theoretical number of PCR products is approximately 2.5x10e9 xG, where G is the size of the haploid genome in base pairs. Using this calculation, a haploid genome of 2x10e9bp, for example, is expected to give about 5 PCR products. This prediction is in close agreement with experimental results. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to working volumes, store at -20 degrees C. Aliquots are stable for 12-12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: R1125-04
Size: 1Kit
References: 1. Grundmann, Towner, Dijkshoorn, Gerner-Smidt, Maher, Seifert, Vaneechoutte. Multicenter study using standardized protocols and reagents for evaluation of reproducibility af PCR-based fingerprinting of Acinetobacter spp. J. Clin. Microbiology 35(12): 3071-3077 (1997). 2. Berg, D., et al., Methods in Molecular and Cellular Biology 5: 13-24 (1994). 3. Welsh, J., McClelland, M., Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18(24): 7213-7218 (1990). 4. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531-6535 (1990). 5. Micheli, M., et al. (1997). In Fingerprinting Methods Based on Arbitrarily Primed PCR, pp. 55-63. Springer-Verlag Press, NY. 6. Graves, L., Swaminathan, B. (1993). In Diagnostic Molecular Microbiology Principles and Applications, pp. 617-621. ASM Press, Washington, DC.

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