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Ras, p21, Recombinant, Human (c-bas/has, c-H-ras, C-HA-RAS1, CTLO, GTPase HRas, HAMSV, Ha-Ras, HRAS, H-Ras, HRAS1, H-Ras-1, H-RASIDX, K-RAS, N-RAS, p21ras, RASH1, Transforming Protein p21, v-Ha-ras Harvey Rat Sarcoma Viral Oncogene Homolog)

Cat no: R1198-01H

Ras, p21, Recombinant, Human (c-bas/has, c-H-ras, C-HA-RAS1, CTLO, GTPase HRas, HAMSV, Ha-Ras, HRAS, H-Ras, HRAS1, H-Ras-1, H-RASIDX, K-RAS, N-RAS, p21ras, RASH1, Transforming Protein p21, v-Ha-ras Harvey Rat Sarcoma Viral Oncogene Homolog)

HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-186 of human HRAS 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG\nCMSCKCLEHH HHHH\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1198-01H

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 0.1M NaCl, 20% glycerol.

Purity

~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. McCormick F. (1995) Mol Reprod Dev. 42(4):500-6 2. AyllOn V. et al. (2000) Mol Membr Biol. 17(2):65-73

Alternative Names

c-has/bas p21

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