RAS Protein (K-ras, N-ras, RASH1, c-bas/has)

The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer. \n\nSource: Recombinant full length human RAS was expressed in E.coli cells using a N-terminal GST tag. \n\nApplications: \nSuitable for use in Kinase Assay. Other applications have not been tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R1198-03

Size

50ug

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~ 90%

References

1. Shih, T. Y., et al: Structure and function of p21 ras proteins. Gene Amplif Anal. 1986;4:53-72. 2. Rodriguez-Viciana, P.: Cancer targets in the Ras pathway. Cold Spring Harb Symp Quant Biol. 2005;70:461-7.