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Ras R2, Recombinant Human (RAS viral (r-ras) oncogene homolog 2, RRAS2)

Cat no: R1200-10G

Ras R2, Recombinant Human (RAS viral (r-ras) oncogene homolog 2, RRAS2)

RAS viral (r-ras) oncogene homolog 2 isoform a, also known as RRAS2, belongs to the Ras like GTPase family that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. It is implicated in the pathogenesis of human cancers. Thus, overexpression of active RRAS2 in EpH4 cells induces tumorigenicity through the phosphoinositide 3-kinase, p38 MAPK, and mTOR pathways, and these cells lose their sensitivity to the normal growth inhibitory role of TGF-beta. Recombinant human RRAS2, fused to His-tag at N-terminus, , expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to human RRAS2, aa1- 201, his-tagged, expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI\nRMNVDQAFHE LVRVIRKFQE QECPPSPEPT RKEKDKKGCH C\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

R1200-10G

Size

100ug

Form

Supplied as liquid in 20mM Tris-HCl buffer, pH8.0, 1mM DTT, 10% glycerol, 2mM EDTA.

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

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