RasGRP4 (RAS Guanyl Releasing Protein 4, RAS Guanyl-releasing Protein 4) (Azide Free)

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. [provided by RefSeq]\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1.5ug/ml\nWestern Blot: 1:500-1:1,000\nELISA: 1ug/ml-3ng/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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