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RASSF1A, Recombinant, Human, aa1-340, His-Tag (Ras Association Domain-containing Protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21)

Cat no: 138726

RASSF1A, Recombinant, Human, aa1-340, His-Tag (Ras Association Domain-containing Protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21)

RASSF1A is similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. This protein required for death receptor-dependent apoptosis. \n\nSource:\nRecombinant protein corresponding to aa1-340 from human RASSF1A at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~42.9kD (376aa)\n\nAA Sequence:\nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSGE PELIELRELA PAGRAGKGRT RLERANALRI ARGTACNPTR QLVPGRGHRF QPAGPATHTW CDLCGDFIWG VVRKGLQCAH CKFTCHYRCR ALVCLDCCGP RDLGWEPAVE RDTNVDEPVE WETPDLSQAE IEQKIKEYNA QINSNLFMSL NKDGSYTGFI KVQLKLVRPV SVPSSKKPPS LQDARRGPGR GTSVRRRTSF YLPKDAVKHL HVLSRTRARE VIEALLRKFL VVDDPRKFAL FERAERHGQV YLRKLLDDEQ PLRLRLLAGP SDKALSFVLK ENDSGEVNWD AFSMPELHNF LRILQREEEE HLRQILQKYS YCRQKIQEAL HACPLG\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138726

Size

100ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

Purity

~85% by (SDS-PAGE)

References

1. Burbee D.G., et al. (2001) J. Natl. Cancer Inst. 93:691-699 2. Shivakumar L., et al. (2002) Mol. Cell. Biol. 22:4309-4318.

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