Rat /Mouse Acrogranin ELISA employs the quantitatively competitive enzyme immunoassay technique in which Rat /Mouse Acrogranin present in samples competed with a fixed amount of biotinylated Acrogranin for sites on purified rabbit IgG specific against Acrogranin. During the incubation, the rabbit IgG becomes bound to the goat anti-rabbit IgG pre-coated onto the microplates. Following a wash to remove any unbound antibody, standard, samples and biotin conjugate, a Streptavidin conjugated to horseradish-peroxidase (HRP) is added to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of Rat /Mouse Acrogranin bound in the initial step. The sample values are then read off the standard curve. Rat /Mouse Acrogranin ELSA has been shown to accurately quantitate natural Rat /Mouse Acrogranin. Results obtained using natural Rat /Mouse Acrogranin showed dose response curves that were parallel to the standard curves obtained using the kit standards.
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