Rat ANP ELISA kit is designed for detection of rat ANP in plasma, serum, tissue extract,and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures ANP in less than 5 hours. A polyclonal antibody specific for rat ANP has been pre-coated onto a microplate. The ANP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for ANP, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured).
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