Rat anti Mouse ENG monoclonal antibody, clone 310832

Rat Anti-Mouse ENG monoclonal antibody for ELISA(Cap), WB. Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin and beta glycan/T beta b RIII are type III receptors for TGF-beta b superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain. A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin. The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta b 1 and TGF-beta b 3 (but not TGF-beta b 2), but only after binding T beta b RII. Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-IA/ALK-3 or BMPR-IB/ALK-6. BMP-9, however, is reported to bind Endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta b 1 signals but enhance BMP-7 signals in the same myoblast cell line. In endothelial cells, Endoglin inhibits T beta b RI/ALK5, but enhances ALK-1-mediated activation. Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization. In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity.

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SPECIFICATIONS

Catalog Number

CABT-L289

Hosts

Rat

Reactivities

Mouse

Conjugates

Unconj

Clone

310832

Target

ENG

Gene Id

13850

Isotype

IgG2A

Clonality

Monoclonal

Storage Temp

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 В°C as supplied. 1 month, 2 to 8 В°C under sterile conditions after reconstitution. 6 months, -20 to -70 В°C under sterile conditions after reconstitution.