rBNP-32 ELISA kit is designed for detection of rat BNP-32 in plasma, serum,tissue extract, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rBNP-32 in less than 5 hours. A polyclonal antibody specific for rBNP-32 has been pre-coated onto a microplate. The rBNP-32 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rBNP-32, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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