Macrophages are cells produced by the differentiation of monocytes in tissues. Macrophages are large cells found within many types of tissue. They derive from monocytes that have entered tissues. Macrophages phagocytise invading microorganisms and also scavenge dead and damaged cells and cellular debris. In the bone marrow and subsequestly in the blood and tissues, cells of this series undergo a series of functional and morphologic maturation steps that culminate in the mature tissue macrophage. Macrogphage can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3 and CD68 by flow cytometory or immunohistochemical staining.
RMa-bm are isolated from adult rat bone marrow tissue. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RMa-bm is characterized by immunofluorescent method with antibody to CD 11b. RMa-bm is negative for mycoplasma, bacteria, yeast and fungi. RMa-bm is guaranteed to further culture in the condition provided by Creative Bioarray.
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