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Rat Spleen Endothelial Cells

Rat Spleen Endothelial Cells

Cat no: CSC-C4133X

Supplier: Creative Bioarray
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Rat Spleen Endothelial Cells from�Creative Bioarray�are isolated from spleen tissue of 6-8 week old laboratory Sprague-Dawley rat. Rat Spleen Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in�Creative Bioarray� Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6�cells per ml and is delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.
Catalogue number: CSC-C4133X
Size: 1.0 mL
Storage temp: Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 �C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 �C.

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