RBL1, CT (RBL1, Retinoblastoma-like protein 1, 107kD retinoblastoma-associated protein, pRb1) (Biotin)

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

040878-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Jayadeva, G., et al. J. Biol. Chem. 285(39):29863-29873(2010)\nLiu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)\nRose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :\nYerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)\nCunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)

Additional Info

Human

Alternative Names

RBL1, Retinoblastoma-like protein 1, 107kD retinoblastoma-associated protein, pRb1