Rcan2, Recombinant, Mouse, aa2-197, His-tag (Calcipressin-2, Calcineurin Inhibitory Protein ZAKI-4, Down Syndrome Candidate Region 1-like Protein 1, Myocyte-enriched Calcineurin-interacting Protein 2, MCIP2, Regulator of Calcineurin 2, Dscr1l1, Zaki4)

Regulator of calcineurin 2 (RCAN2), also known as ZAKI4 and DSCR1L1, is expressed as two isoforms differing at their N-terminus. The longer of the two (isoform 1) is expressed exclusively in the brain, while isoform 2 is ubiquitously expressed, with highest expression in brain, heart, and muscle. Both isoforms bind to the catalytic subunit of calcineurin, a Ca++-dependent protein phosphatase involved in several neuronal functions, though their C-terminal region and inhibit calcineurin's activity. Unlike isoform 1 of RCAN2, the expression of the second isoform is not induced by the thyroid hormone T3. RCAN2 is a member of a family of three endogenous calcineurin regulators that are located near the minimal supernumerary fragment of chromosome 21 in individuals with Down syndrome, suggesting that they play a role in this syndrome.\n\nSource:\nRecombinant protein corresponding to mouse RCAN2 Isoform 2, fused to His-tag at C-terminal expressed in E.coli.\n\nMolecular Weight:\n~26kD\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

149777

Size

50ug

Form

Supplied as a liquid in 100mM sodium phosphate, 10mM Tris, 500mM sodium chloride, 25mM imidazole, 2mM magnesium chloride, 10% glycerol.

Purity

~95%

References

1. Miyazaki T, Kanou Y, Murata Y, et al. Molecular cloning of a novel thyroid hormone-responsive gene, ZAKI-4, in human skin fibroblasts. J. Biol. Chem. 1996; 271:14567-71. 2. Rothermal B, Vega RB, Yang J, et al. A protein encoded within the Down syndrome critical region is enriched in striated muscles and inhibits calcineurin signaling. J. Biol. Chem. 2000; 275:8719-25. 3. Cao X, Kambe F, Miyazaki Y, et al. Novel human ZAKI-4 isoforms: hormonal and tissue-specific regulation and function as calcineurin inhibitors. Biochem. J. 2002; 367:459-66. 4. Fuentes JJ, Genesca L, Kingsbury TJ, et al. DSCR1, overexpressed in Down syndrome, is an inhibitor of calcineurin-mediated signaling pathways. Hum. Mol. Genet. 2000; 9:1681-90.