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RCN3, Recombinant, Human, aa21-328, His-Tag (Reticulocalbin-3, EF-hand Calcium-binding Protein RLP49, UNQ239/PRO272)

Cat no: 045456

RCN3, Recombinant, Human, aa21-328, His-Tag (Reticulocalbin-3, EF-hand Calcium-binding Protein RLP49, UNQ239/PRO272)

Reticulocalbin 3, also known as RCN3, is a member of the CREC (cab45/reticulocalbin/ ERC45/calumenin) family. RCN3 contains five Arg-Xaa-Xaa-Arg motifs, which function as target sequences of SPCs (subtilisin-like proprotein convertases), a family of serine endoproteases that proteolytically activate proproteins. The synthesis of one such member, PACE4 (paired basic amino acid cleaving enzyme 4), is influenced by association and coexpression with reticulocalbin-3.\n\nSource:\nRecombinant corresponding to aa21-328 from human RCN3, fused to His-tag at N-terminus, expressed in E.coli.\n\nMolecular Weight:\n~37.9kD (333aa) confirmed by MALDI-TOF\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSHMKPSPD AGPHGQGRVH QAAPLSDAPH DDAHGNFQYD HEAFLGREVA KEFDQLTPEE SQARLGRIVD RMDRAGDGDG WVSLAELRAW IAHTQQRHIR DSVSAAWDTY DTDRDGRVGW EELRNATYGH YAPGEEFHDV EDAETYKKML ARDERRFRVA DQDGDSMATR EELTAFLHPE EFPHMRDIVI AETLEDLDRN KDGYVQVEEY IADLYSAEPG EEEPAWVQTE RQQFRDFRDL NKDGHLDGSE VGHWVLPPAQ DQPLVEANHL LHESDTDKDG RLSKAEILGN WNMFVGSQAT NYGEDLTRHH DEL\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

045456

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.

Purity

~95% (SDS-PAGE)

References

1. Teglund S., et al. (1994) Genomics. 23:669-684. 2. Tsuji A., et al. (2006) Biochem J. 396:51-59.

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