Receptor Associated Protein, Recombinant, Rat (RAP)

Receptor-associated protein (RAP) functions as a specialized endoplasmic reticulum chaperone assisting in the folding and trafficking of members of the low-density lipoprotein (LDL) receptor family. RAP efficiently binds to these receptors and antagonizes binding of other ligands.\n\nRecombinant Rat Receptor Associated Protein is a fused-Rat his:RAP:c-myc protein with a MW of 40kD (38862 Daltons) as determined by SDS gel electrophoresis.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Human Keratin.\n\nReconstitution:\nReconstitute the lyophilized recombinant RAP in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1283-20

Size

5ug

Form

Supplied as a lyophilized powder in TBS, pH 7.5, 9.5M urea, 0.1% BSA, 0.09% sodium azide.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Efficient transfer of receptor-associated protein (RAP) across the blood-brain barrier. Pan W, Kastin AJ, van Kerkhof P, J Cell Sci 2004 Oct 1;117(Pt 21):5071-8 2. Lipoprotein receptor binding, cellular uptake, and lysosomal delivery of fusions between the receptor-associated protein (RAP) and alpha-L-iduronidase or acid alpha-glucosidase. Prince WS, McCormick LM, Fitzpatrick PA, Christianson TM, J Biol Chem 2004 Aug 13;279(33):35037-46 3. Impaired thyroglobulin (Tg) secretion by FRTL-5 cells transfected with soluble receptor associated protein (RAP): evidence for a role of RAP in the Tg biosynthetic pathway. Lisi S, Chiovato L, Marcocci C, McCluskey RT, J Endocrinol Invest 2003 Nov;26(11):1105-10 4. Mapping of the receptor-associated protein (RAP) binding proteins on living fibroblast cells using an atomic force microscope. Osada T, Itoh A, Ultramicroscopy 2003 Oct-Nov;97(1-4):353-7 5. Dominant thermodynamic role of the third inde pendent receptor binding site in the receptor-associated protein RAP. Andersen OM, Schwarz FP, Jacobsen C, Biochemistry 2001 Dec 18;40(50):15408-17 6. The roles of receptor-associated protein (RAP) as a molecular chaperone for members of the LDL receptor family. Bu G, Int Rev Cytol 2001;209:79-116.