Receptor Tyrosine-protein Kinase ErbB-2, BioAssay(TM) ELISA Kit (Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene C-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL)

The human receptor tyrosine-protein kinase erbB-2 BioAssay(TM) Kit is a sandwich ELISA. The capture antibody is a polyclonal human erbB-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known erbB-2 concentration are added to these wells and allowed to complex with the bound erbB-2 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of erbB-2 present in the samples.\n\nIntended Use:\nThe purpose of this kit is the in-vitro quantitative determination of human erbB-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This BioAssay(TM) kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.\n\nSensitivity:\n<10pg/ml\n\nRange:\n62.5pg/ml-4000pg/ml\n\nKit Components:\nPre-coated strip plates, 2x96wells\nProtein standards\nDetection reagents\nSubstrate buffer\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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