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Recombinant Hepatitis C Virus HCVCB protein

Recombinant Hepatitis C Virus HCVCB protein

Cat no: HCVCB-84H

Supplier: Creative Biomart
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The E.Coli derived 70kDa recombinant protein contains sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then contructed into one recombinant antigen. The protein contains the HCV nucleocapsid, NS3 genotype 1b, NS4 genotype 1b and 1a, and NS5 genotype 1b and 1a immunodominant regions.
Catalogue number: HCVCB-84H
Storage buffer: 50mM Na-PO4, pH-8.5, 2.4mM EDTA, 5mM DTT and 0.1% SDS.
P type: Recombinant protein
Species: Hepatitis C Virus
Purity: HCV protein is >95% pure as determined by 10% PAGE (coomassie staining).
Storage temp: HCV although stable at 4�C for 1 week, should be stored below -18�C. Please prevent freeze thaw cycles.

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